Natalie Ozanne, 28, will be wheel postilion rider in the four-horse team of Windsor Greys that will drive the Queen to the showground in Act 1 Scene 1.
The Queen's 90th Birthday Pageant coincides with the Royal Windsor Horse Show, from today until Sunday.
Over the 90-minute performance, 900 horses and 1,500 participants from around the world will tell the 90-year story, including the excitement of her majesty's birth and her record-breaking 64-year reign.
'My role in this pageant means I'm only the second woman in history to drive the Queen and that is an entire privilege,' said Miss Ozanne, who is a liveried helper for the Royal Mews.